The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
Use at an assay dependent concentration.
1/200. (see Abreview)
ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the band 7/mec-2 family.
Cellular localizationCell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunocytochemistry/ Immunofluorescence - STOM antibody (ab67880)This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa
Central Panel: ab67880 staining STOM in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/250, was used as secondary antibody. Left-hand panel: Nuclei counterstained with DAPI (blue). Right-hand panel: Overlay