Overview

  • Product nameAnti-STOM antibody
    See all STOM primary antibodies
  • Description
    Mouse polyclonal to STOM
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length STOM protein (Human)

  • Positive control
    • STOM transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/200. (see Abreview)



ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the band 7/mec-2 family.
  • Cellular localizationCell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • BND7 antibody
    • EPB7 antibody
    • EPB72 antibody
    • Erythrocyte band 7 integral membrane protein antibody
    • Erythrocyte membrane protein band 7.2 (stomatin) antibody
    • Erythrocyte surface protein band 7.2 antibody
    • Protein 7.2b antibody
    • STOM antibody
    • STOM_HUMAN antibody
    • Stomatin antibody
    see all

Anti-STOM antibody images

  • All lanes : Anti-STOM antibody (ab67880) at 1/500 dilution

    Lane 1 : STOM transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Central Panel: ab67880 staining STOM in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/250, was used as secondary antibody.
    Left-hand panel: Nuclei counterstained with DAPI (blue).
    Right-hand panel: Overlay

    See Abreview

References for Anti-STOM antibody (ab67880)

ab67880 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 30min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100 + 2% BSA in PBS for 15 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"