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Synthetic peptide derived from within residues 1 - 100 of Mouse Stra8.
(Peptide available as ab49601.)
Our Abpromise guarantee covers the use of ab49602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18799790|
ab49602 at 1/250 dilution, staining Stra8 in Primordial Germ Cells from 13.5 days post-coitum mouse ovaries and testis by Immunocytochemistry/ immunofluorescence. The cells were fixed by 4% paraformaldehyde in PBS for 10 min at room temperature and then permeabilized for 10 min in 0.1% Triton X-100 in PBS. After a 1-h block in 5% bovine serum albumin in PBS, primary antibody was added, diluted in 0.5% BSA in PBS and incubated with sample overnight at 4°C. An Alexa Fluor®568 conjugated Goat anti-rabbit IgG was used as secondary for 1 hour. Nuclei were labeled with Hoechst 33349 (1µg/ml). A rabbit IgG was also used as control antibody with the same procedure.
This image is courtesy of an anonymous abreview.
ab49602 staining Stra8 (red) in hamster fetal ovary tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 0.2% Triton X-100 + 10% donkey serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (10µg/ml) was used as the secondary antibody. Nuclei stained blue with DAPI.
Frozen mouse testis tissue stained for Stra8 using ab49602 at 1/3000 dilution in immunohistochemical analysis. Phalloidin (red), which labels F-actin was used as a counterstain.
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