Overview

  • Product nameAnti-Stra8 antibody
    See all Stra8 primary antibodies
  • Description
    Rabbit polyclonal to Stra8
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Mouse Stra8.

    (Peptide available as ab49601.)

  • Positive control
    • This antibody gave a positive signal in CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/250.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18799790

Target

  • FunctionRequired for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase.
  • Tissue specificityExpressed specifically in testis.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • stimulated by retinoic acid 8 homolog antibody
    • Stimulated by retinoic acid Gene 8 antibody
    • stimulated by retinoic acid gene 8 homolog antibody
    • Stimulated by retinoic acid gene 8 protein homolog antibody
    • STRA 8 antibody
    • STRA8 antibody
    • STRA8_HUMAN antibody
    see all

Anti-Stra8 antibody images

  • ab49602 at 1/250 dilution, staining Stra8 in Primordial Germ Cells from 13.5 days post-coitum mouse ovaries and testis by Immunocytochemistry/ immunofluorescence. The cells were fixed by 4% paraformaldehyde in PBS for 10 min at room temperature and then permeabilized for 10 min in 0.1% Triton X-100 in PBS. After a 1-h block in 5% bovine serum albumin in PBS, primary antibody was added, diluted in 0.5% BSA in PBS and incubated with sample overnight at 4°C. An Alexa Fluor®568 conjugated Goat anti-rabbit IgG was used as secondary for 1 hour. Nuclei were labeled with Hoechst 33349 (1µg/ml). A rabbit IgG was also used as control antibody with the same procedure.

  • IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Stra8 antibody (ab49602) at 1 µg/ml

    Lane 1 : CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate
    Lane 2 : CHO Negative Control Whole Cell Lysate

    Lysates/proteins at 1 µl per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
    ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.
  • All lanes : Anti-Stra8 antibody (ab49602) at 1/500 dilution

    Lane 1 : WT D3 mouse ES cells cultured with LIF.
    Lane 2 : WT D3 mouse ES cells cultured without LIF for 24 hours.
    Lane 3 : WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours.

    Lysates/proteins at 200000 cells per lane.

    Secondary
    Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous abreview.

    See Abreview

  • ab49602 staining Stra8 (red) in hamster fetal ovary tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 0.2% Triton X-100 + 10% donkey serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (10µg/ml) was used as the secondary antibody. Nuclei stained blue with DAPI.

    See Abreview

References for Anti-Stra8 antibody (ab49602)

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Busada JT  et al. Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice. Reproduction 152:379-88 (2016). Read more (PubMed: 27486269) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, adult)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, adult
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Apr 12 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Hamster Tissue sections (fetal ovary frozen sections)
Specification fetal ovary frozen sections
Permeabilization Yes - 0.2% Triton x100
Fixative Paraformaldehyde
Username

Prof. SK Roy

Verified customer

Submitted Jun 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

Submitted May 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Loading amount 200000 cells
Specification mouse embryonic stem cell
Treatment 1uM RA for 24 hours
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 30 2012

Merci de nous avoir contactés.

Comme convenu vendredi, j'ai mis en place l'envoi d'une unité de ab49602 en remplacement du ab49405 qui n'a pas fonctionné. Le numéro de commande de remplacement gratuit de ce produit ...

Read More

Merci de nous avoir contactés.

Nous sommes désolés que le produit ab49602 que vous avez reçu ne fonctionne pas comme attendu.

Comme convenu j'ai mis en place l'envoi d'une unité de ab49405. Le num&ea...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Feb 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF, F9, Testis, Kidney)
Loading amount 20 µg
Specification MEF, F9, Testis, Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jul 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"