Overview

  • Product name
    Anti-Strep-tag II antibody
    See all Strep-tag II primary antibodies
  • Description
    Rabbit polyclonal to Strep-tag II
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    tag
  • Immunogen

    Amino acid sequence

    ASWSHPQFEKGA

  • Positive control
    • Streptavidin tagged recombinant protein
  • General notes


    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity chromatography purified via peptide column
  • Primary antibody notes
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml.

Predicted Band Size: 24kDa

Target

  • Relevance
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • Alternative names
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Anti-Strep-tag II antibody images

  • All lanes : Anti-Strep-tag II antibody (ab76949) at 1 µg/ml

    Lane 1 : StreptII tagged recombinant protein at 0.005 µg
    Lane 2 : StrepII tagged recombinant protein at 0.025 µg

References for Anti-Strep-tag II antibody (ab76949)

This product has been referenced in:
  • Ori-McKenney KM  et al. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron 90:551-63 (2016). Read more (PubMed: 27112495) »
  • Borowska MT  et al. A YidC-like Protein in the Archaeal Plasma Membrane. Structure 23:1715-24 (2015). Read more (PubMed: 26256539) »

See all 6 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Jurkat T cells
Blocking step
Western Blotting Reagent (Roche) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Sep 20 2017

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Chicken Cell lysate - whole cell (Lymphocytes)
Specification
Lymphocytes
Total protein in input
100 µg
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Verified customer

Submitted Jan 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Hamster Cell lysate - whole cell (CHO whole cell lysate)
Specification
CHO whole cell lysate
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 23 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (AD293)
Specification
AD293
Treatment
Strep-tagged keratin
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (Strep-tagged recombinant protein)
Loading amount
0.5 µg
Specification
Strep-tagged recombinant protein
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (cell lysate containing expressed recombinant prote)
Loading amount
25 µg
Specification
cell lysate containing expressed recombinant prote
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 03 2012

Vielen Dank für Ihre Anfrage.

Wir haben verschiedene Antikörper die ich Ihnen in diesem Fall empfehlen kann. Die drei Produkte unterscheiden sich in der Hostspezies.

1. Chicken polyclonal Secondary Antibody to Rabbit IgG - H...

Read More

Thank you for your reply.

I have processed your request to receive ab119810 as free of charge replacement for ab76949. Currently, ab119810 is out of stock, but we expect to be able to ship it to you by 11th May, I do apologize for this delay....

Read More

Thank you for your reply.

Both antibodies should work equally as well for you. I was suggested ab119810, as it is the only other Strep tag antibody we have in stock and the only information that we have on it, is what is already on the webpag...

Read More

Thank you for your reply.

I think the best way forward is too either replace or refund the cost of ab76949. An alternative to ab76949 is ab119810:

http://www.abcam.com/Strep-tag-II-antibody-ab119810.html

Please let me know...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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