Overview

  • Product nameAnti-Strep-tag II antibody
    See all Strep-tag II primary antibodies
  • Description
    Rabbit polyclonal to Strep-tag II
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    tag
  • Immunogen

    Amino acid sequence

    ASWSHPQFEKGA

  • Positive control
    • Streptavidin tagged recombinant protein
  • General notes


    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity chromatography purified via peptide column
  • Primary antibody notesStreptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • RelevanceStreptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • Alternative names
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Anti-Strep-tag II antibody images

  • All lanes : Anti-Strep-tag II antibody (ab76949) at 1 µg/ml

    Lane 1 : StreptII tagged recombinant protein at 0.005 µg
    Lane 2 : StrepII tagged recombinant protein at 0.025 µg


    Predicted band size : 24 kDa

References for Anti-Strep-tag II antibody (ab76949)

This product has been referenced in:
  • McCourt JL  et al. In vitro stability of therapeutically relevant, internally truncated dystrophins. Skelet Muscle 5:13 (2015). WB ; Fall armyworm (Spodoptera frugiperda) . Read more (PubMed: 25954502) »
  • Allen CE  et al. Analysis of novel iron-regulated, surface-anchored hemin-binding proteins in Corynebacterium diphtheriae. J Bacteriol 195:2852-63 (2013). Read more (PubMed: 23585541) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Chicken Cell lysate - whole cell (Lymphocytes)
Specification Lymphocytes
Total protein in input 100 µg
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Abcam user community

Verified customer

Submitted Jan 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Hamster Cell lysate - whole cell (CHO whole cell lysate)
Specification CHO whole cell lysate
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 23 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (AD293)
Specification AD293
Treatment Strep-tagged keratin
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (Strep-tagged recombinant protein)
Loading amount 0.5 µg
Specification Strep-tagged recombinant protein
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jan 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (cell lysate containing expressed recombinant prote)
Loading amount 25 µg
Specification cell lysate containing expressed recombinant prote
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 03 2012

Vielen Dank für Ihre Anfrage.

Wir haben verschiedene Antikörper die ich Ihnen in diesem Fall empfehlen kann. Die drei Produkte unterscheiden sich in der Hostspezies.

1. Chicken polyclonal Secondary Antibody to Rabbit IgG - H...

Read More

Thank you for your reply.

I have processed your request to receive ab119810 as free of charge replacement for ab76949. Currently, ab119810 is out of stock, but we expect to be able to ship it to you by 11th May, I do apologize for this delay....

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Thank you for your reply.

Both antibodies should work equally as well for you. I was suggested ab119810, as it is the only other Strep tag antibody we have in stock and the only information that we have on it, is what is already on the webpag...

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Thank you for your reply.

I think the best way forward is too either replace or refund the cost of ab76949. An alternative to ab76949 is ab119810:

http://www.abcam.com/Strep-tag-II-antibody-ab119810.html

Please let me know...

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Thank you for contacting Abcam.

I am sorry about the issue you are having with ab76949 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot. If we cannot resolve the issue y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"