Anti-Strep-tag II antibody (ab76950)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity chromatography purified via peptide column
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • RelevanceStreptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • Alternative names
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Anti-Strep-tag II antibody images

  • All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml

    Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
    Lane 2 : Streptavidin tagged recombinant protein

    Lysates/proteins at 0.05 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References for Anti-Strep-tag II antibody (ab76950)

ab76950 has not yet been referenced specifically in any publications.

Product Wall

We have two different versions of the tag: ab76950- against GGGWSHPQFEK ab76949- against ASWSHPQFEKGA Both contain the tag sequence WSHPQFEK along with extra inert amino acids to make the sequence longer for conjugation chemistry to the carrier ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"