Anti-Strep-tag II antibody (ab76950)
Key features and details
- Rabbit polyclonal to Strep-tag II
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Strep-tag II antibody
See all Strep-tag II primary antibodies -
Description
Rabbit polyclonal to Strep-tag II -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Amino acid sequence gggWSHPQFEK.
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Positive control
- Streptavidin tagged recombinant protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity chromatography purified via peptide column -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa. |
Target
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Relevance
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
Alternative names
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
Images
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All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml
Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
Lane 2 : Streptavidin tagged recombinant protein
Lysates/proteins at 0.05 µg per lane.
Predicted band size: 24 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab76950 has been referenced in 4 publications.
- Reichmann J et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464
- Long T et al. Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition. Nat Commun 10:2452 (2019). PubMed: 31165728
- Baggett NE et al. Global analysis of translation termination in E. coli. PLoS Genet 13:e1006676 (2017). PubMed: 28301469
- Lin Y et al. Antiaging Gene Klotho Deficiency Promoted High-Fat Diet-Induced Arterial Stiffening via Inactivation of AMP-Activated Protein Kinase. Hypertension 67:564-73 (2016). PubMed: 26781278