Anti-Streptavidin antibody [S8E4] (ab10023)

Overview

  • Product nameAnti-Streptavidin antibody [S8E4]
    See all Streptavidin primary antibodies
  • Description
    Mouse monoclonal [S8E4] to Streptavidin
  • SpecificityDoes not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Other, ELISA, Flow Cyt, WBmore details
  • Immunogen

    Recombinant Streptavidin (rSAV).

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone numberS8E4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.

Target

  • RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localizationCytoplasmic
  • Alternative names
    • SA V1 antibody
    • SA V2 antibody
    • strepavidin antibody
    • Streptavidin V1 antibody
    • Streptavidin V2 antibody
    see all

References for Anti-Streptavidin antibody [S8E4] (ab10023)

ab10023 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

I think any of the any of the antibodies you are interested in would work for you in your desired applications. As they are mouse monoclonals, you may see some mouse on mouse staining and unfortunately we do no...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich genauer in den Laboren nachgefragt, die unsere Streptavidin-Antikörper herstellen, und dabei hat sich herausgestellt, dass unsere monoklonalen anti-Streptavidin-Antikörper (ab10020-10023)...

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Thank your for your inquiry.

I can confirm that ab59320 is now unpublished and not available any more. I am sorry if this causes any inconvenience.

ab6676 is a polyclonal antibody that has the whole streptavidin molecule as immuno...

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Thank you for your email. Our source for this antibody supplied me with the information regarding the antibody's affinity. We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to...

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The affinity (Kd) for this antibody is > 1 x 10E8. If you have any additional questions, please contact us again.

Ab10023 was prepared against recombinant streptavidin (rSAV). Unfortunately the direct epitope and sequence of the Mab are not known at the moment. But we know that ab10023 interacts with a SAV-site different from those recognized by ab10022 and ab1002...

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Thank you for your email. Both ab9395 and ab10023 were originated and characterized outside Abcam but the antibodies originate from two different sources. Ab9395 has been tested for application in Western blot, ELISA, and Flow Cytometry / FACS. Ab10023...

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Thank you for your enquiry. Having not tested these antibodies in SELEX or in parallel in other applications it is difficult to compare their quality, my apologies for the inconvenience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"