Overview

  • Product nameStreptavidin Peroxidase (Ready to Use)
  • Tested applicationsSuitable for: IHC-P, Sandwich ELISAmore details
  • General notesSTAINING PROTOCOL:

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Add enough drops of an appropriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.

    3. Perform appropriate pretreatment if required. Wash 3 times in buffer.

    4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.

    5. Apply primary antibody and incubate according to manufacturer's protocol.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary solution according to manufacturer’s protocol. Wash 4 times in buffer.

    7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.

    9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.

Properties

  • RelevanceStreptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

Applications

Our Abpromise guarantee covers the use of ab64269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. PubMed: 21371998

Streptavidin Peroxidase (Ready to Use) images

  • Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).

References for Streptavidin Peroxidase (Ready to Use) (ab64269)

This product has been referenced in:
  • Wilson R  et al. Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLoS One 9:e100572 (2014). Read more (PubMed: 24963654) »
  • Wang Y  et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Read more (PubMed: 21371998) »

See all 2 Publications for this product

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We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Merci de nous avoir contactés.

Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269

...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"