Anti-Streptococcus Enterotoxin A antibody (FITC) (ab20127)

Overview

  • Product name
    Anti-Streptococcus Enterotoxin A antibody (FITC)
  • Description
    Goat polyclonal to Streptococcus Enterotoxin A (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Type specific carbohydrate for group A Streptococcus. No immunological cross reactivity. However, there is potential non-specific reactivity with such organisms as group C Streptococcus and Staphyloccus due to the presence of protein A like proteins (Staphyloccus) and protein C like proteins (Streptococcus C) present on the intact surface of these bacteria. These proteins bind any immunoglobulin (including the FITC Conjugate) and therefore will appear to fluoresce (non-specifically
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with Streptococcus group A. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation.Streptococci, Group A

  • General notes

    Immunoaffinity purified antibody covalently coupled with high purity Isomer I of fluorescein isothiocyanate (F:P ratio=5.4). Care is taken to ensure complete removal of any free fluorescein from the final product.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notes
    ELISA: 1/10 - 1/50.
    IF: 1/10 - 1/50.
    Direct FA staining of target antigens in a permissive tissue culture system. Functions both as capture and detection antibody. Acetone fixation of the antigen source is recommended prior to staining. Pre-block slide with 10% normal goat serum.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nares and pharynx. However, in certain circumstances, they may also inhabit the skin, heart or muscle tissue.
    • Cellular localization
      Cell Membrane

    References

    ab20127 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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