Anti-Streptococcus group A antibody (FITC) (ab20752)

Overview

  • Product name
    Anti-Streptococcus group A antibody (FITC)
    See all Streptococcus group A primary antibodies
  • Description
    Rabbit polyclonal to Streptococcus group A (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Recognizes group A Streptococcus. Does not react with other Strep groups. No immunological cross reactivity. However, there is potential non-specific reactivity with such organisms as group C Streptococcus and Staphyloccus due to the presence of protein A like proteins (Staphyloccus) and protein C like proteins (Streptococcus C) present on the intact surface of these bacteria. These proteins bind any immunoglobulin (including the FITC Conjugate) and therefore will appear to fluoresce (non-specifically).
  • Immunogen

    Streptococci (Group A) cell preparation.

  • General notes

    The product consists of immunoglobulin covalently coupled with high purity isomer I of fluorescein isothiocyanate (F:P ratio=3.2). Care is taken to ensure complete removal of any free fluorescein from the final product.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    IF: 1/10 - 1/50.
    Acetone fixation of the antigen source is recommended prior to staining. Pre-block slide with 10% normal rabbit serum.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nose and pharynx. Streptococci can be divided into many groups on the basis of antigenic differences in group-specific polysaccharides located in the bacterial cell wall. More than 20 serologic groups have been identified and designated by letters, eg, A, B, C. Group A streptococci, cause a wide-range of disease in humans, from mild sore throats to life-threatening invasive disease such as necrotising fasciitis. Streptococcus pyogenes (a group A Streptococcus) is one of the most important pathogens encountered in clinical practice. Group A streptococci (GAS) are gram-positive, nonmotile, non-spore-forming organisms that appear as pairs or short-to-moderate-sized chains. Group A organisms can be identified by enzyme immunoassays.
    • Alternative names
      • Group A strep antibody
      • Group A streptococci antibody
      • Group A streptococcus antibody

    References

    ab20752 has not yet been referenced specifically in any publications.

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