Anti-Streptococcus Group B antibody (ab53584)


  • Product nameAnti-Streptococcus Group B antibody
    See all Streptococcus Group B primary antibodies
  • Description
    Rabbit polyclonal to Streptococcus Group B
  • SpecificityReacts with type specific carbohydrate of group B Streptococcus.
  • Tested applicationsSuitable for: IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with Streptococcus B.
  • Immunogen

    Native Streptococcus Group B.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab53584 was affinity purified.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab53584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • RelevanceStreptococci form part of the normal human flora that resides on the skin, and can also colonise the respiratory, gastrointestinal, and genitourinary tracts. Streptococci can cause a range of diseases, from the less serious but common sore throats and skin infections to life threatening conditions such as necrotising fasciitis. Different streptococcal species are involved in human disease, broadly categorised as pus forming or pyogenic streptococci, non pus forming or non pyogenic streptococci, and Streptococcus pneumoniae. Streptococci are classified into Lancefield serotypes by their cell wall polysaccharide antigens. Group A are primarily pathogens. Group B streptococci (including Streptococcus agalactiae) are the leading bacterial causes of human neonatal illness and death causing opportunistic invasive disease in pregnant women such as preterm labour, membrane rupture and urinary tract infections and sepsis and meningitis in newborns.
  • Alternative names
    • Beta strep antibody
    • Group b streptococci antibody
    • Group b streptococcus antibody
    • Strep b antibody

Anti-Streptococcus Group B antibody images

  • ab53584 staining Streptococcus agalactiae by Immunocytochemistry/ Immunofluorescence.
    Streptococcus agalactiae was infected into fetal rat lung explants for 24 hours. Tissue was fixed and probed "whole-mount". The cells were formaldehyde fixed, permeabilised in 0.1% Triton/1X PBS for 15 minutes and then blocked using 5% Donkey serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for one hour at 25°C. The secondary antibody used was a Donkey anti-rabbit IgG conjugated to FITC used at a 1/400 dilution. Tissue was counterstained with Hoechst 33342 (blue). Image collected using a Leica Microsystems confocal microscope.

    See Abreview

  • ab53584 staining Streptococcus Group B in brain tissue from Oreochromis niloticus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in 10% buffered formalin and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked with 25% serum for 30 minutes at room temperature and then incubated with ab53584 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References for Anti-Streptococcus Group B antibody (ab53584)

This product has been referenced in:
  • Randis TM  et al. Group B Streptococcus ß-hemolysin/Cytolysin Breaches Maternal-Fetal Barriers to Cause Preterm Birth and Intrauterine Fetal Demise in Vivo. J Infect Dis N/A:N/A (2014). Read more (PubMed: 24474814) »
  • Kling DE  et al. Group B Streptococcus induces a caspase-dependent apoptosis in fetal rat lung interstitium. Microb Pathog 61-62C:1-10 (2013). Read more (PubMed: 23624260) »

See all 2 Publications for this product

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ab53584 has been testedin immunocytochemistry (ICC) but not in colloidal gold method.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Fish Tissue sections (Brain from tilapia (Oreochromis niloticus))
Specification Brain from tilapia (Oreochromis niloticus)
Fixative 10% buffered formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: RT°C

Christian Delannoy

Verified customer

Submitted Feb 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Streptococcus agalactiae Cell
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton/1X PBS for 15 minutes
Blocking step Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. David Kling

Verified customer

Submitted Sep 16 2010