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Rabbit polyclonal to Striatin
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human Striatin aa 250-300. The exact sequence is proprietary. (NP_003153.2).
HeLa, 293T, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab193000 was affinity purified using an epitope specific to Striatin immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
Use at 2-10 µg/mg of lysate.
Calmodulin-binding protein which may function as scaffolding or signaling protein and may play a role in dendritic Ca(2+) signaling.
Preferentially expressed in brain.
Belongs to the WD repeat striatin family.
Contains 6 WD repeats.
Cytoplasm. Membrane. Cell projection, dendritic spine. CTTNBP2-binding may regulate dendritic spine distribution.
Information by UniProt
Western blot - Anti-Striatin antibody (ab193000)
All lanes :
Anti-Striatin antibody (ab193000) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lane 4 :
TCMK-1 whole cell lysate
Lane 5 :
NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size:
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-Striatin antibody (ab193000)
Detection of Striatin in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab193000 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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