Overview

  • Product nameAnti-Stromal interaction molecule 1 antibody
    See all Stromal interaction molecule 1 primary antibodies
  • Description
    Rabbit polyclonal to Stromal interaction molecule 1
  • SpecificityAt least two isoforms of Human STIM1 protein are known to exist. ab106531 will detect only the larger of the two isoforms. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region near the carboxy terminus of Human Stromal interaction molecule 1.

  • Positive control
    • Human spleen tissue; Mouse thymus tissue lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 77 kDa.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionPlays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
  • Tissue specificityUbiquitously expressed in various human primary cells and tumor cell lines.
  • Involvement in diseaseDefects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
  • Sequence similaritiesContains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications
    Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

Anti-Stromal interaction molecule 1 antibody images

  • Lane 1 : Anti-Stromal interaction molecule 1 antibody (ab106531) at 1 µg/ml
    Lane 2 : Anti-Stromal interaction molecule 1 antibody (ab106531) at 2 µg/ml

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Mouse thymus tissue lysate


    Predicted band size : 77 kDa
  • ab106531, at 2.5 µg/ml, staining STIM1 in Human spleen tissue by Immunohistochemistry.

References for Anti-Stromal interaction molecule 1 antibody (ab106531)

This product has been referenced in:

See 1 Publication for this product

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