Anti-Stromal interaction molecule 1 antibody (ab57834)

Overview

  • Product name
    Anti-Stromal interaction molecule 1 antibody
    See all Stromal interaction molecule 1 primary antibodies
  • Description
    Mouse monoclonal to Stromal interaction molecule 1
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 24-686 of Human Stromal interaction molecule 1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites 

Properties

Applications

Our Abpromise guarantee covers the use of ab57834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at 4 µg/mg of lysate.

Target

  • Function
    Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
  • Tissue specificity
    Ubiquitously expressed in various human primary cells and tumor cell lines.
  • Involvement in disease
    Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
  • Sequence similarities
    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain
    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications
    Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

Images

  • Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.


  • Predicted band size : 77 kDa
    Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • ab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Li P  et al. Blocking of stromal interaction molecule 1 expression influence cell proliferation and promote cell apoptosis in vitro and inhibit tumor growth in vivo in head and neck squamous cell carcinoma. PLoS One 12:e0177484 (2017). WB . Read more (PubMed: 28494008) »
  • Huang M  et al. The maintenance ability and Ca(2+) availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med 48:e278 (2016). WB . Read more (PubMed: 27932789) »

See all 2 Publications for this product

Customer reviews and Q&As

Any anti-mouse IgG secondary antibody will be a suitable secondary antibody. We have many choices of different conjugations. For an HRP-conjugated secondary, I suggest ab97023, and for a green fluorescent conjugate I suggest Alexa Fluor 488-conjugated ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for calling Abcam earlier today.

I am sorry about the issues you have been having with the current lot of ab57834. If you could provide me with either the Abcam order number or PO# as we discussed, I will be happy to send you a repl...

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Thank you for your reply.

These two antibodies are very similar. Ab57834 is supplied at a size of 100ug with a recommended working dilution in WB of 1:200-1:1000, while ab119288 is supplied at a size of 50ug with a recommended WB dilution of...

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Thank you for contacting us. The Stromal interaction molecule 1 antibody ab57834 was validated using 5% milk as a blocking agent. I hope this helps, please let me know if you need any additional information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
1000 µg
Specification
HeLa
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HELA)
Total protein in input
200 µg
Specification
HELA
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X
Username

Abcam user community

Verified customer

Submitted Aug 19 2010

1-10 of 12 Abreviews or Q&A

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