Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)


  • Product name
    Anti-Stromal interaction molecule 1 antibody [EPR3414]
    See all Stromal interaction molecule 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Stromal interaction molecule 1.

  • Positive control
    • SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab108994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98 &degC, below boiling, and then let cool for 10-20 min.

  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
    • Tissue specificity
      Ubiquitously expressed in various human primary cells and tumor cell lines.
    • Involvement in disease
      Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
    • Sequence similarities
      Contains 1 EF-hand domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain
      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • Post-translational
      Glycosylation is required for cell surface expression.
      Phosphorylated predominantly on Ser residues.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • D11S4896E antibody
      • GOK antibody
      • OTTHUMP00000164512 antibody
      • OTTHUMP00000229140 antibody
      • OTTHUMP00000230742 antibody
      • SIM antibody
      • STIM 1 antibody
      • STIM1 antibody
      • Stim1 stromal interaction molecule 1 antibody
      • STIM1_HUMAN antibody
      • STIM1L antibody
      • Stromal interaction molecule 1 antibody
      see all

    Anti-Stromal interaction molecule 1 antibody [EPR3414] images

    • All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

      Lane 1 : SH SY5Y cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Performed under reducing conditions.

      Predicted band size : 77 kDa
    • ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

    This product has been referenced in:
    • Wu G  et al. Overexpression of stromal interaction molecule 1 may promote epithelial-mesenchymal transition and indicate poor prognosis in gastric cancer. Mol Med Rep 16:151-158 (2017). IHC-P ; Human . Read more (PubMed: 28534934) »
    • Han S  et al. Anti-allergic activity of glycyrrhizic acid on IgE-mediated allergic reaction by regulation of allergy-related immune cells. Sci Rep 7:7222 (2017). Read more (PubMed: 28775294) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab108994.
    Please use the links above to contact us or submit feedback about this product.


    Sign up