Overview

  • Product nameAnti-STT3A antibody [4D4]
    See all STT3A primary antibodies
  • Description
    Mouse monoclonal [4D4] to STT3A
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 603-702 of Human STT3A with proprietary tag (NP_689926).

  • Positive control
    • STT3A transfected 293T cell line lysates; Human Placenta tissue; recombinant tagged STT3A protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number4D4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab116213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionComponent of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity.
  • Tissue specificityExpressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. Expressed in skin fibroblasts (at protein level).
  • Sequence similaritiesBelongs to the STT3 family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B5 antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A antibody
    • FLJ27038 antibody
    • Integral membrane protein 1 antibody
    • Integral transmembrane protein 1 antibody
    • ITM1 antibody
    • MGC9042 antibody
    • Oligosaccharyl transferase subunit STT3A antibody
    • STT 3A antibody
    • STT3 subunit of the oligosaccharyltransferase complex homolog A antibody
    • STT3-A antibody
    • STT3A antibody
    • STT3A_HUMAN antibody
    • TMC antibody
    • Transmembrane protein TMC antibody
    see all

Anti-STT3A antibody [4D4] images

  • All lanes : Anti-STT3A antibody [4D4] (ab116213) at 1/500 dilution

    Lane 1 : 293T cell line lysate, transfected with STT3A
    Lane 2 : 293T cell line lysate, non-transfected


    Predicted band size : 81 kDa
  • ab116213, at 5 µg/ml, staining STT3A in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged STT3A is approximately 1 ng/ml as a capture antibody.

References for Anti-STT3A antibody [4D4] (ab116213)

ab116213 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"