Overview

  • Product nameAnti-STUB1 antibody
    See all STUB1 primary antibodies
  • Description
    Rabbit polyclonal to STUB1
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 253-303 of Human STUB1 (NP_005852.2)

  • Positive control
    • Whole HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionE3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
    • Tissue specificityHighly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 3 TPR repeats.
      Contains 1 U-box domain.
    • DomainThe TPR domain is essential for ubiquitination mediated by UBE2D1.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen NY CO 7 antibody
      • Antigen NY-CO-7 antibody
      • C terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70 interacting protein antibody
      • Carboxy terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70p interacting protein antibody
      • CHIP antibody
      • CHIP_HUMAN antibody
      • CLL associated antigen KW 8 antibody
      • CLL-associated antigen KW-8 antibody
      • E3 ubiquitin protein ligase CHIP antibody
      • E3 ubiquitin-protein ligase CHIP antibody
      • Heat shock protein A binding protein 2 (c terminal) antibody
      • HSPABP2 antibody
      • NY CO 7 antibody
      • PP1131 antibody
      • SDCCAG7 antibody
      • Serologically defined colon cancer antigen 7 antibody
      • STIP1 homology and U Box containing protein 1 antibody
      • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
      • STIP1 homology and U box-containing protein 1 antibody
      • STUB 1 antibody
      • STUB1 antibody
      • UBOX 1 antibody
      • UBOX1 antibody
      see all

    Anti-STUB1 antibody images

    • Detection of Human STUB1 by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab80601 used for IP at 3 mcg/mg lysate. Lane 2: IP with a control IgG. An other antibody anti STUB1 was subsequently used for Western blot detection. Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-STUB1 antibody (ab80601)

    ab80601 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"