Overview

  • Product nameAnti-STUB1 antibody [EPR4448]
    See all STUB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4448] to STUB1
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human STUB1.

  • Positive control
    • HeLa, MCF7, ECV304, and 293T cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionE3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
    • Tissue specificityHighly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 3 TPR repeats.
      Contains 1 U-box domain.
    • DomainThe TPR domain is essential for ubiquitination mediated by UBE2D1.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen NY CO 7 antibody
      • Antigen NY-CO-7 antibody
      • C terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70 interacting protein antibody
      • Carboxy terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70p interacting protein antibody
      • CHIP antibody
      • CHIP_HUMAN antibody
      • CLL associated antigen KW 8 antibody
      • CLL-associated antigen KW-8 antibody
      • E3 ubiquitin protein ligase CHIP antibody
      • E3 ubiquitin-protein ligase CHIP antibody
      • Heat shock protein A binding protein 2 (c terminal) antibody
      • HSPABP2 antibody
      • NY CO 7 antibody
      • PP1131 antibody
      • SDCCAG7 antibody
      • Serologically defined colon cancer antigen 7 antibody
      • STIP1 homology and U Box containing protein 1 antibody
      • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
      • STIP1 homology and U box-containing protein 1 antibody
      • STUB 1 antibody
      • STUB1 antibody
      • UBOX 1 antibody
      • UBOX1 antibody
      see all

    Anti-STUB1 antibody [EPR4448] images

    • All lanes : Anti-STUB1 antibody [EPR4448] (ab109103) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : ECV304 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
    • Immunofluorescent staining of STUB1 in HeLa cells using ab109103 at 1/100 dilution.

    References for Anti-STUB1 antibody [EPR4448] (ab109103)

    This product has been referenced in:
    • El-Kasaby A  et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem 289:28987-9000 (2014). Read more (PubMed: 25202009) »
    • Bergmayr C  et al. Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. J Biol Chem 288:28831-44 (2013). Read more (PubMed: 23965991) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa)
    Total protein in input 1000 µg
    Specification HeLa
    Immuno-precipitation step Protein A/G
    Username

    Abcam user community

    Verified customer

    Submitted Apr 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"