Overview

  • Product name
  • Description
    Rabbit polyclonal to STX19
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (QQAVIYEREP VAERHLHEIQ KLQESINNLA DNVQKFGQQQ KSLVASMRRF) of Human STX19 (NP_001001850).

  • Positive control
    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-STX19 antibody (ab94538) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa

References

ab94538 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Epididymis, Distal colon)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
60 µg
Specification
Epididymis, Distal colon
Blocking step
Odyssey Blocking Buffer (1:1 with TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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