Overview

  • Product nameAnti-Styk1 antibody
    See all Styk1 primary antibodies
  • Description
    Rabbit polyclonal to Styk1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 214-400 of Human Styk1 (NP_060893).

  • Positive control
    • A431 and MOLT4 whole cell lysates; Breast CA; 293T, H1299, HeLaS3 and Raji cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab97451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/250.

Target

  • FunctionProbable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways.
  • Tissue specificityWidely expressed. Highly expressed in brain, placenta and prostate. Expressed in tumor cells such as hepatoma cells LO2, cervix carcinoma cells HeLa, ovary cancer cells Ho8910 and chronic myelogenous leukemia cells K562, but not in other tumor cells such as epidermoid carcinoma (A431). Undetectable in most normal lung tissues, widely expressed in lung cancers.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761P1010 antibody
    • NOK antibody
    • Novel oncogene with kinase domain antibody
    • Protein kinase STYK1 antibody
    • Protein PK unique antibody
    • Protein PK-unique antibody
    • Serine/threonine/tyrosine kinase 1 antibody
    • Styk 1 antibody
    • STYK1 antibody
    • STYK1_HUMAN antibody
    • SuRTK 106 antibody
    • SuRTK106 antibody
    • Tyrosine protein kinase STYK1 antibody
    • Tyrosine-protein kinase STYK1 antibody
    see all

Anti-Styk1 antibody images

  • All lanes : Anti-Styk1 antibody (ab97451) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
  • ab97451 at 1/100 dilution staining Styk1 in paraffin-embedded Breast CA by Immunohistochemistry.

References for Anti-Styk1 antibody (ab97451)

This product has been referenced in:
  • Chen MY  et al. Depletion of STYK1 inhibits intrahepatic cholangiocarcinoma development both in vitro and in vivo. Tumour Biol 37:14173-14181 (2016). Read more (PubMed: 27542675) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 50 µg
Specification 293 cells
Treatment mock tansfection vs. cells transfected with STYK1-FLAG construct
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jun 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"