The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 38 kDa).
Use at an assay dependent dilution.
RelevanceSua7p is the TFIIB of Saccharomyces cerevisiae. The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N-terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance.