Overview

  • Product name
  • Description
    Rabbit polyclonal to Substance P
  • Tested applications
    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Substance P conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Rat spinal cord dorsal horn

Properties

Applications

Our Abpromise guarantee covers the use of ab67006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
  • Application notes
    ICC/IF: 1/300 - 1/500.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant positive labeling of rat substantia nigra and spinal cord using indirect immunofluorescent techniques. Specificity was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.
  • Target

    • Function
      Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
    • Sequence similarities
      Belongs to the tachykinin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-terminal-flanking peptide antibody
      • Hs.2563 antibody
      • neurokinin 1 antibody
      • neurokinin 2 antibody
      • neurokinin A antibody
      • neurokinin alpha antibody
      • Neuromedin L antibody
      • neuropeptide gamma antibody
      • neuropeptide K antibody
      • NK2 antibody
      • NKA antibody
      • NKNA antibody
      • NPK antibody
      • PPT antibody
      • preprotachykinin antibody
      • protachykinin antibody
      • protachykinin-1 antibody
      • Substance K antibody
      • SubstanceP antibody
      • TAC1 antibody
      • TAC2 antibody
      • TAC2, formerly antibody
      • Tachykinin 1 antibody
      • tachykinin 2 antibody
      • tachykinin 2, formerly antibody
      • Tachykinin precursor 1 antibody
      • tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) antibody
      • Tachykinin1 antibody
      • TKN1_HUMAN antibody
      see all

    Images

    • Detection of Substance P in rat spinal cord dorsal horn with ab67006 (25 um sections).

    References

    This product has been referenced in:
    • Herrity AN  et al. The effect of spinal cord injury on the neurochemical properties of vagal sensory neurons. Am J Physiol Regul Integr Comp Physiol 308:R1021-33 (2015). Read more (PubMed: 25855310) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (sural nerve)
    Permeabilization
    No
    Specification
    sural nerve
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ana Leda Bertoncini Simões

    Verified customer

    Submitted Nov 23 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (sural nerve)
    Permeabilization
    No
    Specification
    sural nerve
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Karnovsky adapted
    Username

    Miss. Carolina Giorgetto

    Verified customer

    Submitted Apr 12 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Background Punisher as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Ms. Elisabete de Cassia do Carmo

    Verified customer

    Submitted Aug 17 2015

    ab67006 is designed to recognize the mature or processed form of Substance P.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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