Anti-Substance P antibody [M09205] (ab7340)

Overview

  • Product nameAnti-Substance P antibody [M09205]
    See all Substance P primary antibodies
  • Description
    Rat monoclonal [M09205] to Substance P
  • Tested applicationsSuitable for: IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Substance-P conjugated to BSA.

  • General notes


    Substance P belongs to the tachykinin family. These are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Properties

Applications

Our Abpromise guarantee covers the use of ab7340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21294877

Target

  • FunctionTachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
  • Sequence similaritiesBelongs to the tachykinin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-terminal-flanking peptide antibody
    • Hs.2563 antibody
    • neurokinin 1 antibody
    • neurokinin 2 antibody
    • neurokinin A antibody
    • neurokinin alpha antibody
    • Neuromedin L antibody
    • neuropeptide gamma antibody
    • neuropeptide K antibody
    • NK2 antibody
    • NKA antibody
    • NKNA antibody
    • NPK antibody
    • PPT antibody
    • preprotachykinin antibody
    • protachykinin antibody
    • protachykinin-1 antibody
    • Substance K antibody
    • SubstanceP antibody
    • TAC1 antibody
    • TAC2 antibody
    • TAC2, formerly antibody
    • Tachykinin 1 antibody
    • tachykinin 2 antibody
    • tachykinin 2, formerly antibody
    • Tachykinin precursor 1 antibody
    • tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) antibody
    • Tachykinin1 antibody
    • TKN1_HUMAN antibody
    see all

Anti-Substance P antibody [M09205] images

  • Immunohistochemical analysis of frozen neonatal murine DRG explants, staining Substance P with ab7340 at 1/200 dilution.

    Tissue was fixed in paraformaldehyde, cut into 10 µm sections, permeabilized with 0.1% SDS for 5 mins and incubated in 10% foetal calf serum in TBST for 10 mins. Sections were incubated with primary antibody overnight before incubating with Texas Red-conjugated goat anti-rat secondary antibody (1/250) for 40 minutes at room temperature.

References for Anti-Substance P antibody [M09205] (ab7340)

This product has been referenced in:
  • George L  et al. Familial dysautonomia model reveals Ikbkap deletion causes apoptosis of Pax3+ progenitors and peripheral neurons. Proc Natl Acad Sci U S A 110:18698-703 (2013). Mouse . Read more (PubMed: 24173031) »
  • Wolff G  et al. Neuronal organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus: correspondence with the mushroom body ground pattern. J Comp Neurol 520:2824-46 (2012). Read more (PubMed: 22547177) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (nasal mucosa)
Specification nasal mucosa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

Submitted Jun 10 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step None
Sample Mouse Tissue sections (Trigeminal ganglion)
Specification Trigeminal ganglion
Permeabilization No
Fixative Zamboni
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 10 2015



ab7340 is not a purified antibody, which would typically be suspended in a colorless buffer such as PBS. Rather ab7340 is an unpurified tissue culture supernatant. Tissue culture medium typically has a light purple/pinkish hue so the color yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"