Fluorogenic substrate of calpains and 20S proteasome
Fluorogenic substrate of calpains and 20S proteasome. Calpain I and II hydrolyses Suc-Leu-Leu-Val-Tyr-AMC with Km values of 203 and 466 µM, respectively. Used to study the activity of calpains and proteosomal degradation.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in DMSO to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Wang KK et al. An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective. Proc Natl Acad Sci U S A93:6687-92 (1996).
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Tsubuki S et al. Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome. Biochem Biophys Res Commun196:1195-201 (1993).
Read more (PubMed: 8250877) »
Sasaki T et al. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem259:12489-94 (1984).
Read more (PubMed: 6092335) »