Recombinant
RabMAb

Anti-SUCLA2 antibody [EPR14925] (ab183513)

Overview

  • Product name
    Anti-SUCLA2 antibody [EPR14925]
    See all SUCLA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14925] to SUCLA2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SUCLA2 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9P2R7

  • Positive control
    • Human fetal liver, 293, HepG2 and Molt4 cell lysates, Human breast tissue, HepG2 and 293 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/40 - 1/60.

Target

  • Function
    Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
  • Tissue specificity
    Widely expressed. Not expressed in liver and lung.
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
  • Involvement in disease
    Mitochondrial DNA depletion syndrome 5
  • Sequence similarities
    Belongs to the succinate/malate CoA ligase beta subunit family.
    Contains 1 ATP-grasp domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • A BETA antibody
    • A SCS antibody
    • ATP specific succinyl CoA synthetase subunit beta antibody
    • ATP specific succinyl CoA synthetase, beta subunit antibody
    • ATP-specific succinyl-CoA synthetase subunit beta antibody
    • Mitochondrial succinyl CoA ligase [ADP forming] subunit beta antibody
    • MTDPS5 antibody
    • Renal carcinoma antigen NY-REN-39 antibody
    • Renal carcinoma antigen NYREN39 antibody
    • SCS betaA antibody
    • SCS-betaA antibody
    • SUCB1_HUMAN antibody
    • Succinate CoA ligase (ADP forming) antibody
    • Succinate CoA ligase [ADP forming] subunit beta, mitochondrial; succinyl CoA ligase [ADP forming] subunit beta, mitochondrial antibody
    • Succinate CoA ligase ADP forming beta subunit antibody
    • Succinate CoA ligase beta subunit antibody
    • Succinyl CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl CoA synthetase beta A chain antibody
    • Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl-CoA synthetase beta-A chain antibody
    • SUCLA2 antibody
    see all

Images

  • Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Flow cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

  • Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

  • All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Molt4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 50 kDa

References

ab183513 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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