Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (?= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
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Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
|Enzyme Mix (lyophilized)||Green||1 vial|
|Invertase (Lyophilized)||Blue1||1 vial|
|OxiRed Probe in DMSO||Red||1 x 200µl|
|Sucrose Assay Buffer||WM||1 x 25ml|
|Sucrose Standard (Lyophilised)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab83387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab83387 has not yet been referenced specifically in any publications.