Overview

  • Product nameSucrose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 µM
  • Range
    0 µM - 10 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (?= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enzyme Mix (lyophilized) Green 1 vial
    Invertase (Lyophilized) Blue1 1 vial
    OxiRed Probe in DMSO Red 1 x 200µl
    Sucrose Assay Buffer WM 1 x 25ml
    Sucrose Standard (Lyophilised) Yellow 1 x 100µl
  • Research areas
  • RelevanceSucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
  • Alternative names
    • Saccharose
    • Table sugar

Applications

Our Abpromise guarantee covers the use of ab83387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Sucrose Assay Kit images

  • Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.

Protocols

References for Sucrose Assay Kit (ab83387)

ab83387 has not yet been referenced specifically in any publications.

Product Wall

The laboratory has reassured me that mannitol will not be detected. You will still want to include a negative control that leaves out the invertase, though, and subtract this value from the values of the samples that do receive invertase.

The laboratory confirmed that the buffer should be stable for six months from the date of shipment if stored and handled correctly.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"