Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/25000 - 1/115000. This product has been assayed against 1µg of Sucrose Phosphorylase in a standard capture ELISA using Peroxidase conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSucrose phosphorylase enzyme catalyzes breakdown of sucrose to form fructose and glucose 1-phosphate. Sucrose phosphorylase is involved in the intracellular catabolism of sucrose and the production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.