Overview

  • Product nameAnti-SUGT1 antibody
    See all SUGT1 primary antibodies
  • Description
    Rabbit polyclonal to SUGT1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 315-365 of Human SUGT1 (NP_001124384.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
  • Sequence similaritiesBelongs to the SGT1 family.
    Contains 1 CS domain.
    Contains 1 SGS domain.
    Contains 3 TPR repeats.
  • DomainThe CS domain mediates interaction with HSP90.
  • Post-translational
    modifications
    Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein 40 6 3 antibody
    • Protein 40-6-3 antibody
    • Protein SGT1 homolog antibody
    • Putative 40 6 3 protein antibody
    • Sgt1 antibody
    • SGT1 suppressor of G2 allele of SKP1 antibody
    • SGT1_HUMAN antibody
    • SGT1B protein antibody
    • sugt1 antibody
    • Suppressor of G2 allele of SKP1 homolog antibody
    • Suppressor of G2 allele of SKP1 S. cerevisiae homolog of antibody
    see all

Anti-SUGT1 antibody images

  • All lanes : Anti-SUGT1 antibody (ab104253) at 0.1 µg/ml

    Lane 1 : HeLa whole cell line lysate at 50 µg
    Lane 2 : HeLa whole cell line lysate at 15 µg
    Lane 3 : HeLa whole cell line lysate at 5 µg
    Lane 4 : 293T whole cell line lysate at 50 µg
    Lane 5 : NIH3T3 whole cell line lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 30 seconds
  • Detection of SUGT1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab104253 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-SUGT1 antibody (ab104253)

ab104253 has not yet been referenced specifically in any publications.

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I can confirm that Anti-SUGT1 antibody (ab104253) has been tested, and it is therefore guaranteed to work in Western Blot with the species mentioned on the datasheet (mouse and human).

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