Overview

  • Product name
  • Description
    Rabbit polyclonal to SUGT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Chimpanzee, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human SUGT1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_001124384.1
    Sequence:

    MAAAAAGTAT SQRFFQSFSD ALIDEDPQAA LEELTKALEQ KPDDAQYYCQ


    Database link: Q9Y2Z0

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
  • Sequence similarities
    Belongs to the SGT1 family.
    Contains 1 CS domain.
    Contains 1 SGS domain.
    Contains 3 TPR repeats.
  • Domain
    The CS domain mediates interaction with HSP90.
  • Post-translational
    modifications
    Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein 40 6 3 antibody
    • Protein 40-6-3 antibody
    • Protein SGT1 homolog antibody
    • Putative 40 6 3 protein antibody
    • Sgt1 antibody
    • SGT1 suppressor of G2 allele of SKP1 antibody
    • SGT1_HUMAN antibody
    • SGT1B protein antibody
    • sugt1 antibody
    • Suppressor of G2 allele of SKP1 homolog antibody
    • Suppressor of G2 allele of SKP1 S. cerevisiae homolog of antibody
    see all

Images

  • All lanes : Anti-SUGT1 antibody (ab176849) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 30 seconds
  • Detection of SUGT1 by Western Blot of Immunoprecipitate.
    ab176849 at 0.4 µg/ml labeling SUGT1 in HeLa whole cell lysate immunoprecipitated using ab176849 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds

References

ab176849 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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