This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19797313

Target

  • FunctionExhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.
  • Tissue specificityExpressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, breast, pancreatic, renal and hepatocellular carcinoma cell lines.
  • Sequence similaritiesBelongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localizationEndoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • DSulf antibody
    • Extracellular sulfatase Sulf 1 antibody
    • Extracellular sulfatase Sulf-1 antibody
    • Extracellular sulfatase Sulf1 antibody
    • HSULF 1 antibody
    • HSulf-1 antibody
    • Neu5 antibody
    • Sulf1 antibody
    • SULF1_HUMAN antibody
    • Sulfatase FP antibody
    • Sulfated antibody
    see all

References for Anti-Sulfatase 1 antibody (ab32763)

This product has been referenced in:
  • Cheriyan VT  et al. Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis. PLoS One 9:e93711 (2014). WB ; Human . Read more (PubMed: 24690739) »
  • Ratelade J  et al. A murine model of Denys-Drash syndrome reveals novel transcriptional targets of WT1 in podocytes. Hum Mol Genet 19:1-15 (2010). IHC-Fr ; Mouse . Read more (PubMed: 19797313) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Casein in PBS as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Sample Human Tissue sections (Kidney)
Specification Kidney
Permeabilization Yes - 0.25% Triton X-100
Fixative Formaldehyde
Username

Dr. Francesca Conserva

Verified customer

Submitted Jun 06 2013

Thank you for contacting us yesterday and for sharing the problems you have been experiencing with the Anti-Sulfatase 1 antibody (ab32763).
This antibody is a Fast Tranck antibody and as such has as yet not been tested in anything other than ELISA...

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Merci de nous avoir contactés.

Comme indiqué sur la fiche technique (http://www.abcam.com/ab32763), cet anti-Sulfatase 1 est un produit "fast-track", c'est à dire que c'est un produit qui n'a été ca...

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Thank you for filling the questionnaire.

The protocol seems fine however there is only one suggestion we would like to offer; heating the lysates for 10 minutes at 100C may improve the results.

This protein is very heavily glycosyla...

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Thank you for your email. I am sorry to hear that the customer is having trouble using this antibody.

The link you have provided does not show any information about the cell line so could you ask customer to fill the attached questionnaire. <...

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Application Western blot
Sample Human Cell lysate - whole cell (MCF7 HEK293 786-O)
Loading amount 20 µg
Specification MCF7 HEK293 786-O
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Dec 23 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Muscle, oral squamous cell carcinoma tissues)
Specification Muscle, oral squamous cell carcinoma tissues
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO Cytomation Target Retrival Solution
Blocking step DAKO Cytomation Protein Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 13 2009

Application Western blot
Sample Human Cell lysate - whole cell (lung alveolar type II)
Loading amount 30 µg
Specification lung alveolar type II
Treatment shRNA treated for a period of 11 days
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"