The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Use at an assay dependent concentration. Detects a band of approximately 100 kDa. ab113405 detects a major band of approximately 50kDa in Sulfatase 2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.
FunctionExhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
Tissue specificityExpressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
Sequence similaritiesBelongs to the sulfatase family.
Post-translational modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Cellular localizationEndoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.