Overview

  • Product name
    Anti-Sulfatase 2 antibody [2B4]
    See all Sulfatase 2 primary antibodies
  • Description
    Mouse monoclonal [2B4] to Sulfatase 2
  • Specificity
    ab113405 shows no cross-reactivity with Sulfatase 1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant Human Sulfatase 2.

  • Epitope
    ab113405 recognises an epitope located in the C terminal domain of Sulfatase 2.
  • Positive control
    • Sulfatase 2 transfected HEK29 cells or non-small-cell Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab113405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa. ab113405 detects a major band of approximately 50kDa in Sulfatase 2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.

Target

  • Function
    Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • Tissue specificity
    Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
  • Sequence similarities
    Belongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localization
    Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Extracellular sulfatase Sulf 2 antibody
    • Extracellular sulfatase Sulf-2 antibody
    • HSulf-2 antibody
    • HSulf2 antibody
    • Sulf2 antibody
    • SULF2_HUMAN antibody
    see all

Images

  • Paraffin embedded HEK293 cells transfected with Sulfatase 2 stained with ab113405.

References

This product has been referenced in:
  • Lemjabbar-Alaoui H  et al. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene 29:635-46 (2010). Read more (PubMed: 19855436) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T Transfected with SULF2 over expressing pl)
Permeabilization
Yes - .1% Triton x-100 in 1XPBS with 1 % Goat Serum
Specification
HEK 293T Transfected with SULF2 over expressing pl
Blocking step
5% Goat serum in 1XPBS with 0.01% Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Jul 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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