Overview

  • Product nameAnti-Sulfatase 2 antibody
    See all Sulfatase 2 primary antibodies
  • Description
    Rabbit polyclonal to Sulfatase 2
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: NPRTRNMDLG LKDGGSYEQY RQFQRRKWPE MKRPSSKSLG QLWEGWEG, corresponding to a region within C terminal amino acids 823-870 of Human Sulfatase 2

Properties

Applications

Our Abpromise guarantee covers the use of ab86368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 98 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionExhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • Tissue specificityExpressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
  • Sequence similaritiesBelongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localizationEndoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Extracellular sulfatase Sulf 2 antibody
    • Extracellular sulfatase Sulf-2 antibody
    • HSulf-2 antibody
    • HSulf2 antibody
    • Sulf2 antibody
    • SULF2_HUMAN antibody
    see all

Anti-Sulfatase 2 antibody images

  • Anti-Sulfatase 2 antibody (ab86368) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (1:50,000-100,000)

    Predicted band size : 98 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    This antibody recognises a splice variant of Sulfatase 2.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Sulfatase 2 with ab86368  at 4-8ug/ml. Arrows indicate positively labelled epithelial cells of the renal tubule.

References for Anti-Sulfatase 2 antibody (ab86368)

This product has been referenced in:
  • Zhang H  et al. HSULF-1 inhibits ERK and AKT signaling and decreases cell viability in vitro in human lung epithelial cells. Respir Res 13:69 (2012). WB . Read more (PubMed: 22873647) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"