Recombinant
RabMAb

Anti-SULT2A1 antibody [EPR16095] - N-terminal (ab190690)

Overview

  • Product name
    Anti-SULT2A1 antibody [EPR16095] - N-terminal
    See all SULT2A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR16095] to SULT2A1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SULT2A1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q06520

  • Positive control
    • HepG2 and human adrenal gland tissue lysate. Human liver tissue. HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
  • Tissue specificity
    Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

Images

  • All lanes : Anti-SULT2A1 antibody [EPR16095] - N-terminal (ab190690) at 1/10000 dilution

    Lane 1 : HepG2 cell line lysate
    Lane 2 : Human adrenal gland tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunofluorescent analysis of  4% paraformaldehyde labeling SULT2A1 with ab190690 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 and counterstained with DAPI.

  • Immunohistochemical analysis of paraffin embedded human liver labeling SULT2A1 with ab190690 at 1/500 followed by HRP conjugated secondary antibody and counterstained with hematoxylin.

References

ab190690 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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