Recombinant
RabMAb

Anti-SULT2A1 antibody [EPR16096] (ab194113)

Overview

  • Product name
    Anti-SULT2A1 antibody [EPR16096]
    See all SULT2A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR16096] to SULT2A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SULT2A1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06520

  • Positive control
    • HepG2, Human fetal liver, Human adrenal gland, mouse spleen, rat kidney and rat liver lysates; Human adrenal tissue; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
  • Tissue specificity
    Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

Images

  • All lanes : Anti-SULT2A1 antibody [EPR16096] (ab194113) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human adrenal gland lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SULT2A1 antibody [EPR16096] (ab194113) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab194113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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