Overview

  • Product nameAnti-SULT2B1 antibody
    See all SULT2B1 primary antibodies
  • Description
    Rabbit polyclonal to SULT2B1
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (

    YSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFI

    ) of Human SULT2B1 (NP_004596).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay is 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
  • Tissue specificityExpressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
  • Sequence similaritiesBelongs to the sulfotransferase 1 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alcohol sulfotransferase antibody
    • EC 2.8.2.2 antibody
    • HSST2 antibody
    • Hydroxysteroid sulfotransferase 2 antibody
    • ST2B1 antibody
    • ST2B1_HUMAN antibody
    • Sulfotransferase 2B1 antibody
    • Sulfotransferase family cytosolic 2B member 1 antibody
    • SULT2B1 antibody
    • SULT2B1a antibody
    • SULT2B1b antibody
    see all

Anti-SULT2B1 antibody images

  • Anti-SULT2B1 antibody (ab82865) at 1 µg/ml (5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Anti-SULT2B1 antibody (ab82865) at 1/1000 dilution + whole cell lysate prepared from murine macrophages at 30 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-SULT2B1 antibody (ab82865)

This product has been referenced in:
  • Segala G  et al. 5,6-Epoxy-cholesterols contribute to the anticancer pharmacology of Tamoxifen in breast cancer cells. Biochem Pharmacol 86:175-89 (2013). Read more (PubMed: 23500540) »
  • Yang X  et al. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo. PLoS One 8:e60853 (2013). WB, ICC/IF ; Mouse, Human . Read more (PubMed: 23593328) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (liver cells)
Specification liver cells
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Loading amount 30 µg
Specification macrophages
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"