Anti-Sumo 2 antibody - Carboxyterminal end (ab53194)

Overview

  • Product nameAnti-Sumo 2 antibody - Carboxyterminal end
    See all Sumo 2 primary antibodies
  • Description
    Rabbit polyclonal to Sumo 2 - Carboxyterminal end
  • SpecificityThe immunogen sequence is identical in many species including rat, mouse, pig, and bovine Sumo 2 and is identical in Human Sumo 3.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    DEDTIDVFQQQTGG

    conjugated to KLH, corresponding to C terminal amino acids 80-93 of Human Sumo 2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 10 - 20 µg/ml.

Target

  • FunctionUbiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
  • Tissue specificityBroadly expressed.
  • Sequence similaritiesBelongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.
    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
  • Cellular localizationNucleus. Nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSMT 3 antibody
    • HSMT3 antibody
    • MGC117191 antibody
    • Sentrin 2 antibody
    • Sentrin-2 antibody
    • Sentrin2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • Small ubiquitin-related modifier 2 antibody
    • SMT 3B antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3, yeast, homolog 2 antibody
    • Smt3A antibody
    • SMT3B antibody
    • SMT3H2 antibody
    • SUMO-2 antibody
    • SUMO-3 antibody
    • SUMO2 antibody
    • SUMO2_HUMAN antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3B antibody
    • Ubiquitin-like protein SMT3A antibody
    see all

References for Anti-Sumo 2 antibody - Carboxyterminal end (ab53194)

ab53194 has not yet been referenced specifically in any publications.

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