Overview

  • Product nameAnti-Sumo 2+3 antibody
    See all Sumo 2+3 primary antibodies
  • Description
    Rabbit polyclonal to Sumo 2+3
  • Specificity

    This antibody recognizes Human Sumo 2 and Sumo 3.

    This antibody shows no cross-reactivity with related proteins SUMO1, UCRP, Nedd8, and FAT10 in Western blotting.

  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    MSEEKPKEGVKTEND

    , corresponding to N terminal amino acids 1-15 of Human Sumo 2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purification notesThis antibody has been partially purified by salt precipitation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa). Dilute to working strength with 50mM sodium phosphate buffer (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Target

  • RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
  • Database links
  • Alternative names
    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    see all

Anti-Sumo 2+3 antibody images



  • Predicted band size : 12 kDa


    SUMOylation assay utilising His6-tagged SUMO 2, SUMO E1, SUMO E2 and RANGAP1 as substrate in presence (lane 2) and absence (lanes (1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with ab22654.
  • IHC image of ab22654 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22654, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab22654 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22654, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Sumo 2+3 antibody (ab22654)

This product has been referenced in:
  • Conn KL  et al. Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. J Virol 90:4807-26 (2016). Read more (PubMed: 26937035) »
  • Sabò A  et al. SUMOylation of Myc-family proteins. PLoS One 9:e91072 (2014). WB ; Human . Read more (PubMed: 24608896) »

See all 2 Publications for this product

Product Wall

The peptide sequence used to generate the antibody is highly conserved between SUMO-2 and SUMO-3. The antibody has been tested for reactivity against both SUMO-2 and SUMO-3 and reacts with both proteins equally well.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"