This antibody recognizes Human Sumo 2 and Sumo 3.
This antibody shows no cross-reactivity with related proteins SUMO1, UCRP, Nedd8, and FAT10 in Western blotting.
Our Abpromise guarantee covers the use of ab22654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa). Dilute to working strength with 50mM sodium phosphate buffer (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"