Overview

  • Product nameAnti-Sumo 2+3 antibody [AT10F1]
    See all Sumo 2+3 primary antibodies
  • Description
    Mouse monoclonal [AT10F1] to Sumo 2+3
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus Monkey
  • Immunogen

    Recombinant Human Sumo 2 (1-93aa; MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGG) purified from E. coli.

  • Positive control
    • HeLa cell lysate; Recombinant Sumo 2 or Sumo 3 protein; HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberAT10F1
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab104123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 12 kDa.
ELISA 1/250.
ICC/IF 1/250 - 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
  • Database links
  • Alternative names
    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    see all

Anti-Sumo 2+3 antibody [AT10F1] images

  • All lanes : Anti-Sumo 2+3 antibody [AT10F1] (ab104123) at 1/500 dilution

    Lane 1 : Hela cell lysate at 35 µg
    Lane 2 : Recombinant Sumo 2 at 0.01 µg
    Lane 3 : Recombinant Sumo 3 at 0.01 µg

    Secondary
    Goat anti-mouse HRP conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
  • Immunofluorescence staining of Sumo 2+3 in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104123 (1/500) with Texas Red (Red).
  • ab104123 staining Sumo 2+3 in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.

References for Anti-Sumo 2+3 antibody [AT10F1] (ab104123)

ab104123 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104123.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"