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Mouse monoclonal [AT10F1] to Sumo 2+3
Predicted to work with:
Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey
Recombinant Human Sumo 2 (1-93aa; MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGG) purified from E. coli.
HeLa cell lysate; Recombinant Sumo 2 or Sumo 3 protein; HeLa cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Protein A purified
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500. Predicted molecular weight: 12 kDa.
1/250 - 1/500.
Use at an assay dependent concentration.
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.
Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cytoplasmic (SUMO3) and Nuclear (SUMO2)
Western blot - Sumo 2+3 antibody [AT10F1] (ab104123)
All lanes : Anti-Sumo 2+3 antibody [AT10F1] (ab104123) at 1/500 dilution Lane 1 : Hela cell lysate at 35 µg Lane 2 : Recombinant Sumo 2 at 0.01 µg Lane 3 : Recombinant Sumo 3 at 0.01 µg Secondary All lanes : Goat anti-mouse HRP conjugate Developed using the ECL technique. Predicted band size: 12 kDa
Immunocytochemistry/ Immunofluorescence - Sumo 2+3 antibody [AT10F1] (ab104123)
Immunofluorescence staining of Sumo 2+3 in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104123 (1/500) with Texas Red (Red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sumo 2+3 antibody [AT10F1] (ab104123)
ab104123 staining Sumo 2+3 in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"