Recombinant
RabMAb

Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196)

Overview

  • Product name
    Anti-Sumo 2+3 antibody [EPR300(2)]
    See all Sumo 2+3 primary antibodies
  • Description
    Rabbit monoclonal [EPR300(2)] to Sumo 2+3
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human Sumo 2+3.

  • Positive control
    • Paraffin-embedded Human ovarian tissue HeLa and Jurkat cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR300(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
    • Cellular localization
      Cytoplasmic (SUMO3) and Nuclear (SUMO2)
    • Database links
    • Alternative names
      • HSMT3 antibody
      • MGC117191 antibody
      • OTTHUMP00000115275 antibody
      • OTTHUMP00000115276 antibody
      • OTTHUMP00000115277 antibody
      • Sentrin 2 antibody
      • Small ubiquitin like modifier 2 antibody
      • Small ubiquitin related modifier 2 antibody
      • small ubiquitin-like modifier 3 antibody
      • small ubiquitin-related modifier 3 antibody
      • SMT3 homolog 1 antibody
      • SMT3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 3 antibody
      • SMT3A antibody
      • SMT3B antibody
      • SMT3H1 antibody
      • SMT3H2 antibody
      • Sumo2 antibody
      • Sumo3 antibody
      • Ubiquitin like protein SMT3A antibody
      • Ubiquitin like protein SMT3B antibody
      see all

    Anti-Sumo 2+3 antibody [EPR300(2)] images

    • All lanes : Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 12 kDa
    • ab109196, at 1/100, staining Sumo 2+3 in paraffin-embedded Human ovarian tissue by Immunohistochemistry.

    References for Anti-Sumo 2+3 antibody [EPR300(2)] (ab109196)

    ab109196 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon carcinoma xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    colon carcinoma xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    10% NBF
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    colon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    10% NBF
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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