Recombinant Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sumo 2 + Sumo 3 antibody [EPR4602]
See all Sumo 2 + Sumo 3 primary antibodies -
Description
Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, 293T, SW480 and HL60 cell lysates; Human colonic and hepatocellular carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4602 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
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ICC/IF | (1) |
1/400.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
ICC/IF
1/400. |
Target
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Relevance
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them. -
Cellular localization
Cytoplasmic (SUMO3) and Nuclear (SUMO2) -
Database links
- Entrez Gene: 6612 Human
- Entrez Gene: 6613 Human
- Entrez Gene: 170930 Mouse
- Entrez Gene: 20610 Mouse
- Entrez Gene: 499417 Rat
- Entrez Gene: 690244 Rat
- Omim: 602231 Human
- Omim: 603042 Human
see all -
Alternative names
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : SW480 cell lysate
Lane 5 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab109005 has been referenced in 11 publications.
- Wang L et al. SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1. Kidney Dis (Basel) 8:424-435 (2022). PubMed: 36466072
- Wang Y et al. STUB1 is targeted by the SUMO-interacting motif of EBNA1 to maintain Epstein-Barr Virus latency. PLoS Pathog 16:e1008447 (2020). PubMed: 32176739
- Gómez-Molina C et al. Small Extracellular Vesicles in Rat Serum Contain Astrocyte-Derived Protein Biomarkers of Repetitive Stress. Int J Neuropsychopharmacol 22:232-246 (2019). PubMed: 30535257
- Carmichael RE et al. Insulin-dependent GLUT4 trafficking is not regulated by protein SUMOylation in L6 myocytes. Sci Rep 9:6477 (2019). PubMed: 31019221
- Heras G et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). PubMed: 29868881