Overview

  • Product nameAnti-Sumo 2+3 antibody [EPR4602]
    See all Sumo 2+3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4602] to Sumo 2+3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Sumo 2+3 aa 1 to the C-terminus.

  • Positive control
    • HeLa, Jurkat, 293T, SW480 and HL60 cell lysates; Human colonic and hepatocellular carcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Sumo 2+3 antibody (Alexa Fluor® 488) [EPR4602] (ab196330)
    Anti-Sumo 2+3 antibody (Alexa Fluor® 647) [EPR4602] (ab196578)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4602
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/400.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
    • Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
    • Database links
    • Alternative names
      • HSMT3 antibody
      • MGC117191 antibody
      • OTTHUMP00000115275 antibody
      • OTTHUMP00000115276 antibody
      • OTTHUMP00000115277 antibody
      • Sentrin 2 antibody
      • Small ubiquitin like modifier 2 antibody
      • Small ubiquitin related modifier 2 antibody
      • small ubiquitin-like modifier 3 antibody
      • small ubiquitin-related modifier 3 antibody
      • SMT3 homolog 1 antibody
      • SMT3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 3 antibody
      • SMT3A antibody
      • SMT3B antibody
      • SMT3H1 antibody
      • SMT3H2 antibody
      • Sumo2 antibody
      • Sumo3 antibody
      • Ubiquitin like protein SMT3A antibody
      • Ubiquitin like protein SMT3B antibody
      see all

    Anti-Sumo 2+3 antibody [EPR4602] images

    • Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Sumo 2+3 antibody [EPR4602] (ab109005) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SW480 cell lysate
      Lane 5 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
    • ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-Sumo 2+3 antibody [EPR4602] (ab109005)

    This product has been referenced in:
    • Huang CJ  et al. The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes. Sci Rep 5:15600 (2015). Mouse . Read more (PubMed: 26493771) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"