Overview

  • Product name
  • Description
    Rabbit polyclonal to Sumo 3
  • Specificity

    The immunogen has 96% homology to Human SUMO 2.

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. There is no crossreactivity with Human SUMO 1, yeast SUMO, Arabidopsis thaliana SUMO 1,Arabidopsis thaliana SUMO 2, Tomato SUMO.

  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Human SUMO 3

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/2000. Detects a band of approximately 11.6 kDa (predicted molecular weight: 11 kDa).

Target

  • Function
    Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
  • Tissue specificity
    Expressed predominantly in liver.
  • Sequence similarities
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Polymeric chains can be formed through Lys-11 cross-linking.
    Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Small ubiquitin like modifier 3 antibody
    • Small ubiquitin related modifier 3 antibody
    • Small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3, yeast, homolog 1 antibody
    • SMT3A antibody
    • Smt3B antibody
    • SMT3H1 antibody
    • SUMO-2 antibody
    • SUMO-3 antibody
    • sumo3 antibody
    • SUMO3_HUMAN antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin-like protein SMT3B antibody
    see all

Images

  • All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (5% non-fat dry milk in TTBS)

    Lane 1 : Human GFP SUMO 3 at 1 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
    Lane 2 : Human GFP SUMO 3 at 3 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
    Lane 3 : Human GFP SUMO 3 at 5 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
    Lane 4 : Human GFP SUMO 3 at 10 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS

    Secondary
    Donkey anti rabbit IgG at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11.6 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (in 5% non-fat dry milk in TTBS)

    Lane 1 : MW marker
    Lane 2 : GFP human SUMO 3 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
    Lane 3 : GFP human SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
    Lane 4 : GFP yeast SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
    Lane 5 : GFP Arabidopsis thaliana SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
    Lane 6 : GFP Arabidopsis thaliana SUMO 2 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
    Lane 7 : GFP tomato SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS

    Secondary
    Donkey anti-rabbit IgG at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11.6 kDa (why is the actual band size different from the predicted?)

References

ab34661 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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