Overview

  • Product name
  • Description
    Rabbit polyclonal to SUMO4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SUMO4 aa 49-63 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    CEPRGLSMKQIRFRFG


    Database link: Q6EEV6

  • Positive control
    • Human breast carcinoma and hepatocarcinoma tissues, HepG2 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 11 kDa.

Target

  • Function
    Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.
  • Tissue specificity
    Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.
  • Sequence similarities
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ281H8.4 antibody
    • IDDM5 antibody
    • Small ubiquitin like modifier 4 protein antibody
    • Small ubiquitin-like protein 4 antibody
    • Small ubiquitin-related modifier 4 antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antibody
    • SMT3H4 antibody
    • SUMO 4 antibody
    • SUMO-4 antibody
    • SUMO4 antibody
    • SUMO4_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling SUMO4 using ab170850 at a 1/50 dilution, followed by peroxidase-conjugation of the secondary antibody and AEC staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue, labeling SUMO4 using ab170850 at a 1/50 dilution, followed by peroxidase-conjugation of the secondary antibody and DAB staining.

  • Anti-SUMO4 antibody (ab170850) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size: 11 kDa

References

ab170850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170850.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up