Recombinant Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163]
See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies -
Description
Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Sumo 2 + Sumo 3 + Sumo 4 aa 50-150 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region in Sumo 4 that has significant homology with Sumo 2 and Sumo 3.
Database link: Q6EEV6 -
Positive control
- HepG3, 293T and Jurkat whole cell lysate (ab7899); Human spleen tissue lysate; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.82 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7163 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa).
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ICC/IF |
1/250 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa). |
ICC/IF
1/250 - 1/500. |
Images
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Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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ab126606 at 1/250 dilution staining Sumo 2 + Sumo 3 + Sumo 4 in HepG2 (Human liver hepatocellular carcinoma cell line) cells by immunofluorescence.
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All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa -
All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/5000 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab126606 has been referenced in 4 publications.
- Osmanovic A et al. A SUMO4 initiator codon variant in amyotrophic lateral sclerosis reduces SUMO4 expression and alters stress granule dynamics. J Neurol 269:4863-4871 (2022). PubMed: 35503374
- Liang JX et al. SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells. Oncol Lett 20:225 (2020). PubMed: 32968447
- Ma X et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970
- Tan F et al. Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia. Mol Med Rep 17:1283-1288 (2018). PubMed: 29115559