Recombinant
RabMAb

Anti-SUMO4 antibody [EPR7163] (ab126606)

Overview

  • Product name
    Anti-SUMO4 antibody [EPR7163]
    See all SUMO4 primary antibodies
  • Description
    Rabbit monoclonal [EPR7163] to SUMO4
  • Specificity
    The blast search of immunogen shows high sequence homology with SUMO2 and SUMO3 proteins. Hence ab126606 is more likely to cross react with these proteins however it hasn't been experimentally tested yet.
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SUMO4.

  • Positive control
    • HepG3, 293T and Jurkat cell lysates; Human spleen tissue lysate; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.
    • Tissue specificity
      Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.
    • Sequence similarities
      Belongs to the ubiquitin family. SUMO subfamily.
      Contains 1 ubiquitin-like domain.
    • Post-translational
      modifications
      In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.
    • Information by UniProt
    • Database links
    • Alternative names
      • dJ281H8.4 antibody
      • IDDM5 antibody
      • Small ubiquitin like modifier 4 protein antibody
      • Small ubiquitin-like protein 4 antibody
      • Small ubiquitin-related modifier 4 antibody
      • SMT3 suppressor of mif two 3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antibody
      • SMT3H4 antibody
      • SUMO 4 antibody
      • SUMO-4 antibody
      • SUMO4 antibody
      • SUMO4_HUMAN antibody
      see all

    Images

    • Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab126606 at 1/250 dilution staining SUMO4 in HepG2 cells by immunofluorescence.
    • All lanes : Anti-SUMO4 antibody [EPR7163] (ab126606) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 11 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126606 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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