Overview

  • Product nameAnti-SUN1 antibody
    See all SUN1 primary antibodies
  • Description
    Mouse polyclonal to SUN1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SUN1 (AAH13613, 1 a.a. ~ 258 a.a) full-length human protein.

  • Positive control
    • SUN1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 90 kDa).

Target

  • FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
  • Sequence similaritiesContains 1 SUN domain.
  • DomainThe SUN domain may play a role in the nuclear anchoring and/or migration.
  • Cellular localizationNucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein unc-84 homolog A antibody
    • Sad1 and UNC84 domain containing 1 antibody
    • Sad1/unc 84 protein like 1 antibody
    • Sad1/unc-84 protein-like 1 antibody
    • Sun domain-containing protein 1 antibody
    • Sun1 antibody
    • SUN1_HUMAN antibody
    • UNC 84 homolog A (C elegans) antibody
    • UNC 84A antibody
    • UNC84 A antibody
    • UNC84 antibody
    • UNC84 homolog A (C elegans) antibody
    • UNC84A antibody
    see all

Anti-SUN1 antibody images

  • All lanes : Anti-SUN1 antibody (ab67999) at 1/500 dilution

    Lane 1 : SUN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 90 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-SUN1 antibody (ab67999)

ab67999 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"