Overview

  • Product nameAnti-SUN1 antibody [EPR6554]
    See all SUN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6554] to SUN1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human SUN1.

  • Positive control
    • Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
    • Sequence similaritiesContains 1 SUN domain.
    • DomainThe SUN domain may play a role in the nuclear anchoring and/or migration.
    • Cellular localizationNucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein unc-84 homolog A antibody
      • Sad1 and UNC84 domain containing 1 antibody
      • Sad1/unc 84 protein like 1 antibody
      • Sad1/unc-84 protein-like 1 antibody
      • Sun domain-containing protein 1 antibody
      • Sun1 antibody
      • SUN1_HUMAN antibody
      • UNC 84 homolog A (C elegans) antibody
      • UNC 84A antibody
      • UNC84 A antibody
      • UNC84 antibody
      • UNC84 homolog A (C elegans) antibody
      • UNC84A antibody
      see all

    Anti-SUN1 antibody [EPR6554] images



    • Performed under reducing conditions.

      Predicted band size : 90 kDa


      Exposure time : 7 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    • All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 90 kDa
    • ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

    References for Anti-SUN1 antibody [EPR6554] (ab124770)

    This product has been referenced in:
    • Chow KH  et al. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell 25:160-8 (2014). Human . Read more (PubMed: 24196834) »

    See 1 Publication for this product

    Product Wall

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 36 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification HeLa, RPE-1
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (skin)
    Loading amount 10 µg
    Specification skin
    Treatment SiRNA SUN1for 48h
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS)
    Loading amount 20 µg
    Specification U2OS
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Andrew Cobb

    Verified customer

    Submitted Jul 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"