The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notesIs unsuitable for IP.
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
Sequence similaritiesContains 1 SUN domain.
DomainThe SUN domain may play a role in the nuclear anchoring and/or migration.
ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.
References for Anti-SUN1 antibody [EPR6554] (ab124770)
This product has been referenced in:
Chow KH et al. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell25:160-8 (2014).
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