Overview

  • Product nameAnti-SUN2 antibody
    See all SUN2 primary antibodies
  • Description
    Rabbit polyclonal to SUN2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide derived from within residues 650 to the C-terminus of Human SUN2.

  • Positive control
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab65447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceSUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
    • Cellular localizationNuclear membrane, endosome membrane, mitotic spindle organization.
    • Database links
    • Alternative names
      • FRIGG antibody
      • KIAA0668 antibody
      • nuclear envelope protein antibody
      • Protein unc-84 homolog B antibody
      • Rab5 interacting protein antibody
      • RAB5IP antibody
      • Sad1 and UNC84 domain containing 2 antibody
      • Sad1 unc-84 domain protein 2 antibody
      • Sad1 unc84 domain protein antibody
      • Sad1/unc-84 protein-like 2 antibody
      • Sad1/unc84 protein-like antibody
      • SUN domain-containing protein 2 antibody
      • UNC 84B antibody
      • unc84 homolog B (C. elegans) antibody
      • unc84 homolog B antibody
      • unc84, C. elegans, homolog of, B antibody
      • UNC84B antibody
      see all

    Anti-SUN2 antibody images

    • Anti-SUN2 antibody (ab65447) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 80 kDa
      Observed band size : 80 kDa


      Exposure time : 1 minute
    • IHC image of Ab65447 staining in Human Cervical Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab65447, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • All lanes : Anti-SUN2 antibody (ab65447) at 1 µg/ml

      Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
      Lane 2 : Heart (Mouse) Tissue Lysate
      Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 80 kDa
      Observed band size : 80 kDa


      Exposure time : 1 minute

    References for Anti-SUN2 antibody (ab65447)

    ab65447 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Sep 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"