The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 80 kDa.
SUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
Saunders CA et al. TorsinA controls TAN line assembly and the retrograde flow of dorsal perinuclear actin cables during rearward nuclear movement. J Cell Biol216:657-674 (2017).
Read more (PubMed: 28242745) »
Gutiérrez-Fernández A et al. Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid. EMBO J34:1875-88 (2015).
Read more (PubMed: 25991604) »