Recombinant
RabMAb

Anti-SUN2 antibody [EPR6557] (ab124916)

Overview

  • Product nameAnti-SUN2 antibody [EPR6557]
    See all SUN2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6557] to SUN2
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of Human SUN2.

  • Positive control
    • WB: Human fetal muscle, Saos-2, HeLa, Jurkat and HepG2 lysates. IHC-P: Human lung and ovary tissues. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-SUN2 antibody (HRP) [EPR6557] (ab198987)
    Anti-SUN2 antibody (Alexa Fluor® 488) [EPR6557] (ab198981)
    Anti-SUN2 antibody (Alexa Fluor® 647) [EPR6557] (ab198985)

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.

 

IHC-P 1/250 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

 

ICC/IF 1/100 - 1/800.

 

Target

  • RelevanceSUN proteins form part of the LINC complex - a protein bridge that spans the nuclear envelope linking the nucleoskeleten to the actin cytoskeleten. They are located on the inner nuclear membrane side of the complex. The LINC complex is thought to function in controlling nuclear position, contributing to mechanical resistance and the overall architecture of the cell. SUN2 can exist in a heterodimer with SUN1. Both can interact with lamins and nesprins in the nuclear envelope.
  • Cellular localizationNuclear membrane, endosome membrane, mitotic spindle organization.
  • Database links
  • Alternative names
    • FRIGG antibody
    • KIAA0668 antibody
    • nuclear envelope protein antibody
    • Protein unc-84 homolog B antibody
    • Rab5 interacting protein antibody
    • RAB5IP antibody
    • Sad1 and UNC84 domain containing 2 antibody
    • Sad1 unc-84 domain protein 2 antibody
    • Sad1 unc84 domain protein antibody
    • Sad1/unc-84 protein-like 2 antibody
    • Sad1/unc84 protein-like antibody
    • SUN domain-containing protein 2 antibody
    • UNC 84B antibody
    • unc84 homolog B (C. elegans) antibody
    • unc84 homolog B antibody
    • unc84, C. elegans, homolog of, B antibody
    • UNC84B antibody
    see all

Anti-SUN2 antibody [EPR6557] images

  • ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 80 kDa
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SUN2 (red) with ab124916 at a 1/30 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An  HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Mouse heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 80 kDa
  • ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

  • All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution

    Lane 1 : HeLa cell Lysate
    Lane 2 : Jurkat cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 80 kDa
  • ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

  • All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal muscle lysate
    Lane 2 : Saos-2 lysate
    Lane 3 : HeLa lysate
    Lane 4 : Jurkat lysate
    Lane 5 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 80 kDa
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-SUN2 antibody [EPR6557] (ab124916)

This product has been referenced in:
  • Donahue DA  et al. SUN2 Overexpression Deforms Nuclear Shape and Inhibits HIV. J Virol 90:4199-214 (2016). Flow Cyt . Read more (PubMed: 26865710) »
  • Stewart RM  et al. Nuclear-cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. J Cell Biol 209:403-18 (2015). IHC-P, ICC/IF . Read more (PubMed: 25963820) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (heart tissue lysate)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Loading amount 40 µg
Specification heart tissue lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Submitted Jun 19 2017

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (Vascular smooth muscle cell)
Specification Vascular smooth muscle cell
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Oct 23 2013

ab124916 tested positive on HepG2 cells in ICC, however, the laboratory did not save the image.

A quick literature search may help for potential targets within the LINC complex that associate with F-actin. I performed a quick search and found...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"