Overview

  • Product nameAnti-SUOX antibody
    See all SUOX primary antibodies
  • Description
    Mouse polyclonal to SUOX
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein

  • Positive control
    • WB: liver or HepG2 cell lysate. IHC: kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Suggested method: place sample in 1X citrate buffer pH 6.0, microwave at 750W for 20 minutes, let sample cool down.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • PathwayEnergy metabolism; sulfur metabolism.
    • Involvement in diseaseDefects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD) [MIM:272300]; also known as sulfocysteinuria. ISOD is characterized by neurological abnormalities including multicystic leukoencephalopathy with brain atrophy. Patients often suffer from seizures. Often leads to death at an early age.
    • Sequence similaritiesContains 1 cytochrome b5 heme-binding domain.
    • Cellular localizationMitochondrion intermembrane space.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 1.8.3.1 antibody
      • mitochondrial antibody
      • Sulfite oxidase antibody
      • Sulfite oxidase mitochondrial antibody
      • Sulfite oxidase, mitochondrial precursor antibody
      • Suox antibody
      • SUOX_HUMAN antibody
      see all

    Anti-SUOX antibody images

    • Anti-SUOX antibody (ab88346) at 1 µg/ml + Human liver lysate at 25 µg

      Secondary
      Goat anti-Mouse IgG at 1/5000 dilution

      Predicted band size : 60 kDa
    • Anti-SUOX antibody (ab88346) at 1 µg/ml + HepG2 cell lysate at 25 µg

      Secondary
      Goat anti-Mouse IgG at 1/5000 dilution

      Predicted band size : 60 kDa
    • All lanes : Anti-SUOX antibody (ab88346) at 1 µg/ml

      Lane 1 : SUOX transfected 293T cell lysate
      Lane 2 : Untransfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-Mouse IgG at 1/5000 dilution

      Predicted band size : 60 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    • Immunoperoxidase staining of SUOX in formalin-fixed, paraffin-embedded Human kidney using purified ab88346 at 3 µg/ml. Antigen retrieval was performed by placing sample in 1X citrate buffer pH 6.0 and microwaving at 750W for 20 minutes.

    References for Anti-SUOX antibody (ab88346)

    ab88346 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"