Anti-Superoxide Dismutase 1 antibody (ab183881)

Overview

  • Product name
    Anti-Superoxide Dismutase 1 antibody
    See all Superoxide Dismutase 1 primary antibodies
  • Description
    Rabbit polyclonal to Superoxide Dismutase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment within Human Superoxide Dismutase 1 aa 75-154. The exact sequence is proprietary.
    Database link: P00441

  • Positive control
    • NT2D1 , PC-3 , U87-MG , SK-N-SH , HeLa , Rat brain lysates, Hela cells, colon carcinoma, muscle, rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab183881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities
    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Images

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC-3 whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • Immunocytochemical analysis of methanol fixed Hela cells labeling Superoxide Dismutase 1 at 1/100 dilution with ab183881.  Bottom panel shows co-staining with Hoechst 33342 (blue)

  • Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded rat brain labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution

  • Immunohistochemical analysis of paraffin-embedded Muscle labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution

  • Immunohistochemical staining of paraffin-embedded colon carcinoma labeling Superoxide Dismutase 1 at 1/500 dilution with ab183881

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution

    Lane 1 : Hela mock control
    Lane 2 : HeLa transfected shSuperoxide Dismutase 1

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa



    15% SDS-PAGE

References

ab183881 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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